Bioremediating Butuanon: Investigating the Potential of Termite Nest Microbes and Banana Peel Biochar in Improving Water Quality
DOI:
https://doi.org/10.5281/zenodo.19183900Keywords:
termite nest microbes, banana peel biochar, water quality, butuanon riverAbstract
Termite nest microbes and banana peel biochar are natural, nutrient-rich materials with potential applications in sustainable bioremediation. This study evaluated their effectiveness as low-cost treatment agents for improving the water quality of the critically polluted Butuanon River in Mandaue City, Cebu. Using a posttest-only true experimental design under a quantitative research approach, polluted river water samples were treated with termite nest microbes and banana peel biochar prepared via pyrolysis at 350°C applied individually and in combination at varying concentrations (1g, 2g, and 3g). The effects of the treatments were assessed based on changes in pH level, turbidity, odor, color, and selected heavy metal concentrations across three trials. Water samples were collected from the field and analyzed under controlled laboratory conditions during the academic year 2024–2025. Statistical analysis using one-way ANOVA and Tukey HSD post hoc tests revealed that all treatments significantly altered water parameters (p < 0.05). The findings indicate that banana peel biochar demonstrated stronger performance in enhancing clarity, color, and odor due to its high adsorption capacity and porous structure. In contrast, combined treatments produced greater, dose-dependent increases in pH levels, attributed to the synergistic buffering effect of mineral-rich termite material and alkaline biochar. However, higher combined dosages also increased turbidity, likely due to suspended particles from the added materials. While no heavy metals were detected, the presence of non-metal contaminants like sulfite and fluoride was noted. Overall, the results highlight the potential of termite nest microbes and banana peel biochar as effective, low-cost, and environmentally sustainable alternatives for water quality improvement. These findings support the application of locally available natural materials in community-based environmental remediation efforts.
References
Abdus-Salam, N., & Bello, M. O. (2015). Kinetics, thermodynamics and competitive adsorption of lead and zinc ions onto termite mound. International Journal of Environmental Science and Technology, 13(2), 733–742. https://doi.org/10.1007/s13762-015-0769-2
Adebajo, S. O., Akintokun, P. O., Ezaka, E., Ojo, A. E., Olannye, D. U., & Ayodeji, O. D. (2021). Use of termitarium soil as a viable source for biofertilizer and biocontrol. Bulletin of the National Research Centre, 45(1), Article 110. https://doi.org/10.1186/s42269-021-00560-8
Akpomie, K. G., & Conradie, J. (2020). Banana peel as a biosorbent for the decontamination of water pollutants: A review. Environmental Chemistry Letters, 18(6), 1085–1112. https://doi.org/10.1007/s10311-020-00995-x
Al-Sareji, O. J., Grmasha, R. A., Meiczinger, M., Al-Juboori, R. A., Somogyi, V., & Hashim, K. S. (2024). A sustainable banana peel activated carbon for removing pharmaceutical pollutants from different waters: Production, characterization, and application. Materials, 17(5), Article 1032. https://doi.org/10.3390/ma17051032
Altman, D. G. (1991). Practical statistics for medical research. Chapman and Hall.
Apandi, N. M., Muhamad, M. S., Yek, T. W., Sunar, N. M., & Nagarajah, R. (2023). Activated banana peel macrocomposite adsorbent for river water treatment. Water Practice & Technology, 18(4), 753–770. https://doi.org/10.2166/wpt.2023.050
Apori, S. O., Murongo, M., Hanyabui, E., Atiah, K., & Byalebeka, J. (2020). Potential of termite mounds and surrounding soils as soil amendments. BMC Research Notes, 13(1), Article 371. https://doi.org/10.1186/s13104-020-05236-6
Brunauer, S., Emmett, P. H., & Teller, E. (1938). Adsorption of gases in multimolecular layers. Journal of the American Chemical Society, 60(2), 309–319. https://doi.org/10.1021/ja01269a023
Chamen, C. K., & Visco, E. S. (2024). Watershed management and rehabilitation of Butuanon River. Journal of Human Ecology and Sustainability, 2(1), 1–17. https://doi.org/10.56237/jhes2301
Choudhury, T. R., Acter, T., Uddin, N., Kamal, M., Sarwaruddin Chowdhury, A. M., & Safiur Rahman, M. (2021). Heavy metals contamination of river water and sediments in the mangrove forest ecosystems in Bangladesh: A consequence of oil spill incident. Environmental Nanotechnology, Monitoring & Management, 16, Article 100484. https://doi.org/10.1016/j.enmm.2021.100484
Enagbonma, B. J., & Babalola, O. O. (2019). Environmental sustainability: A review of termite mound soil material and its bacteria. Sustainability, 11(14), Article 3847. https://doi.org/10.3390/su11143847
Hutchinson, G. E. (1957). Concluding remarks. Cold Spring Harbor Symposia on Quantitative Biology, 22, 415–427. https://doi.org/10.1101/SQB.1957.022.01.039
Kusumadewi, R. A., Ali, F., Laksono, S., Putra, N., Fathoni, A. M., Rezqi, K., & Mahlia, T. M. I. (2025). Organic adsorbents for removing dissolved organic matter (DOM): Toward low-cost water purification. Water, 17(16), Article 2433. https://doi.org/10.3390/w17162433
Lee, D. J., Cheng, Y. L., Wong, R. J., & Wang, X. D. (2018). Adsorption removal of natural organic matters in waters using biochar. Bioresource Technology, 260, 413–416. https://doi.org/10.1016/j.biortech.2018.04.016
Meng, Z., Bai, X., & Tang, X. (2022). Short-term assessment of heavy metals in surface water from Xiaohe River irrigation area, China: Levels, sources and distribution. Water, 14(8), Article 1273. https://doi.org/10.3390/w14081273
Montgomery, D. C. (2017). Design and analysis of experiments (9th ed.). John Wiley & Sons.
Opafola, O. T., David, A. O., Ajibade, F. O., Adelodun, B., Alabi, O. O., Olayanju, A., & Aladejana, J. T. (2022). The utilization of bentonite enhanced termite mound clay for the adsorption of methylene blue dye from aqueous solution. Applied Water Science, 12(1), Article 9. https://doi.org/10.1007/s13201-021-01462-5
Tukey, J. W. (1949). Comparing individual means in the analysis of variance. Biometrics, 5(2), 99–114. https://doi.org/10.2307/3001913
Zar, J. H. (2010). Biostatistical analysis (5th ed.). Pearson Education.






